Protein Details: Calcium-activated chloride channel regulator 2

Protein ID

ICDB_Pro_1210

Protein Name

Calcium-activated chloride channel regulator 2

Gene Name

Clca2; Clca5

Organism

Mus musculus (Mouse)

Length

942 amino acids

AlphaFoldDB

AF-Q8BG22-F1-model_v4.pdb

Function

Plays a role in modulating chloride current across the plasma membrane in a calcium-dependent manner; and cell adhesion. Involved in basal cell adhesion and/or stratification of squamous epithelia. May act as a tumor suppressor in breast and colorectal cancer. Plays a key role for cell adhesion in the beginning stages of lung metastasis via the binding to ITGB4.

Sequence

MTHRDSTGPVIGLKLVTLLFTLSPELLFLGAGLKLKENGYDGLLVAINPRVPEDLKLITNIKEMITEASFYLFNATKRRVFFRNVQILVPATWTDHNYSRVRQESYDKANVIVAEQSEEHGDDPYTLQHRGCGQEGRYIHFTPSFLLNDELAAGYGARGRVFVHEWAHLRWGVFDEYNNDKPFYVNGRNEIQVTRCSSDITGVFVCEKGLCPHEDCIISKIFREGCTFLYNSTQNATGSIMFMPSLPSVVEFCNESTHNQEAPNLQNQVCSLRSTWDVITASSDLNHSLPVHGVGLPAPPTFSLLQAGDRVVCLVIDVSRKMAEGDRLLRLQQAAELYLMQVVEAHTFVGIVTFDSKGEIRASLQQIYSDDDRKLLVSYLPTAVSTDAETNICAGVKKGFEVVEERNGRADGSVLILVTSGADEHIANCLLTSMNSGSTIHSMALGSSAARKVGELSRLTGGLKFFIPDKFTSNGMTEAFVRISSGTGDIFQQSLQVESVCETVQPQHQLADTMTVDSAVGNDTLFLVTWQTGGPPEIALLDPSGRKYNTGDFIINLAFRTASLKIPGTAKHGHWTYTLNNTHHSPQALKVTVASRASSLAMSPATLEAFVERDSTYFPQPVIIYANVRKGLHPILNATVVATVEPEAGDPVVLQLLDGGAGADVIRNDGIYSRYFSSFAVSGSYSLTVHVRHSPSTSTLALPVPGNHAMYVPGYITNDNIQMNAPKNLGHRPVKERWGFSRVSSGGSFSVLGVPDGPHPDMFPPCKITDLEAMKVEDDVVLSWTAPGEDFDQGQTTSYEIRMSRSLWNIRDDFDNAILVNSSELVPQHAGTRETFTFSPKLVTHELDHELAEDAQEPYIVYVALRAMDRSSLRSAVSNIALVSMSLPPNSSPVVSRDDLILKGVLTTVGLIAILCLIMVVAHCIFNRKKRPSRKENETKFL

PDB Structures

Ligand Binding

1. DICL_CP

2. DICL_Pep

Binding Site

BINDING 164; /ligand="Zn(2+)"; /ligand_note="catalytic"; BINDING 168; /ligand="Zn(2+)"; /ligand_note="catalytic"; BINDING 175; /ligand="Zn(2+)"; /ligand_note="catalytic";

Disease

Location

Highly expressed in eye; spleen; lung; kidney; uterus; and endothelial cells. Weakly expressed in heart and throughout the gastrointestinal tract. Highly expressed in mammary cell lines. Highly expressed in mammary cell lines. Highly expressed in mammary cell lines. Its expression in immortalized cell line HC11 correlates with slow or arrested growth. Re-expression in mammary tumor cells reduces colony survival.

DOI ID

10.1074/jbc.m408334200; 10.1074/jbc.m408354200; 10.1126/science.1112014; 10.1101/gr.2596504

RefSeq

NP_848812.1 [Q8BG22-1]; XP_006501498.1